LINCS L1000
The LINCS L1000 is a high-throughput, reduced representation gene expression profiling assay developed as part of the NIH Library of Integrated Network-Based Cellular Signatures (LINCS) Program. L1000 directly measures 978 landmark genes and computationally infers the expression of 11,350 additional genes, enabling cost-effective large-scale transcriptional profiling at approximately $2 per sample. The technology was developed to create a Connectivity Map (CMap) that catalogs cellular responses to genetic and chemical perturbations, facilitating drug discovery, mechanism of action determination, and functional annotation of genetic variants. The LINCS L1000 dataset comprises over 1.3 million gene expression profiles representing responses to 19,811 chemical compounds, genetic perturbations targeting 5,075 genes (via shRNA knockdowns and cDNA overexpression), and 314 biologics across multiple cell lines and time points.
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