Eukaryotic Phosphorylation Site Database 2.0
EPSD (Eukaryotic Phosphorylation Site Database) is a comprehensive database that curates and annotates experimentally identified protein phosphorylation sites across eukaryotic species. Version 2.0 contains 2,769,163 phosphorylation sites in 362,707 phosphoproteins from 223 eukaryotic species (95 animals, 20 protists, 61 plants, 48 fungi). The database integrates data from high-throughput phosphoproteomic studies and 10 public databases, providing detailed annotations including phosphopeptide sequences, localization probability scores, cell/tissue sources, and primary references. EPSD 2.0 features 88,074 functional annotations for 32,762 phosphorylation sites, covering 58 types of downstream effects on phosphoproteins and 107 regulatory impacts on biological processes. For eight model organisms (human, mouse, rat, Drosophila, C. elegans, Arabidopsis, S. pombe, S. cerevisiae), phosphoproteins are meticulously annotated with information from 100 external resources across 15 aspects including kinase/phosphatase regulators, 3D structures, physicochemical characteristics, genomic variations, functional descriptions, protein domains, molecular interactions, drug-target associations, disease data, orthologs, transcript expression, proteomics, subcellular localization, and regulatory pathways. EPSD 2.0 represents a 2.5-fold increase in data volume compared to version 1.0 and provides intrinsic disorder propensity and surface accessibility calculations for phosphorylation sites. The database supports advanced search options including substrate search, peptide search, batch search, BLAST search, and browse by species functionality.
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