Eukaryotic Phosphorylation Site Database 2.0
EPSD (Eukaryotic Phosphorylation Site Database) is a comprehensive database that curates and annotates experimentally identified protein phosphorylation sites across eukaryotic species. Version 2.0 contains 2,769,163 phosphorylation sites in 362,707 phosphoproteins from 223 eukaryotic species (95 animals, 20 protists, 61 plants, 48 fungi). The database integrates data from high-throughput phosphoproteomic studies and 10 public databases, providing detailed annotations including phosphopeptide sequences, localization probability scores, cell/tissue sources, and primary references. EPSD 2.0 features 88,074 functional annotations for 32,762 phosphorylation sites, covering 58 types of downstream effects on phosphoproteins and 107 regulatory impacts on biological processes. For eight model organisms (human, mouse, rat, Drosophila, C. elegans, Arabidopsis, S. pombe, S. cerevisiae), phosphoproteins are meticulously annotated with information from 100 external resources across 15 aspects including kinase/phosphatase regulators, 3D structures, physicochemical characteristics, genomic variations, functional descriptions, protein domains, molecular interactions, drug-target associations, disease data, orthologs, transcript expression, proteomics, subcellular localization, and regulatory pathways. EPSD 2.0 represents a 2.5-fold increase in data volume compared to version 1.0 and provides intrinsic disorder propensity and surface accessibility calculations for phosphorylation sites. The database supports advanced search options including substrate search, peptide search, batch search, BLAST search, and browse by species functionality.
[]
[{"category": "Portal", "description": "Main web portal for EPSD 2.0 providing search, browse, and visualization of protein phosphorylation sites across 223 eukaryotic species with functional annotations", "format": "http", "id": "epsd.portal", "name": "EPSD 2.0 Portal", "original_source": ["epsd"], "product_url": "https://epsd.biocuckoo.cn", "warnings": ["File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by SSLError(SSLCertVerificationError(1, '[SSL_ CERTIFICATE_VERIFY_FAILED] certificate verify failed_ certificate has expired (_ssl.c_1017)')))", "File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by NewConnectionError('<urllib3.connection.HTTPSConnection object at 0x7fd6e7ba18b0>_ Failed to establish a new connection_ [Errno 111] Connection refused'))", "File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by NewConnectionError('<urllib3.connection.HTTPSConnection object at 0x7fe0f52798d0>_ Failed to establish a new connection_ [Errno 111] Connection refused'))", "File was not able to be retrieved when checked on 2025-11-25_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by SSLError(SSLCertVerificationError(1, '[SSL_ CERTIFICATE_VERIFY_FAILED] certificate verify failed_ certificate has expired (_ssl.c_1000)')))"]}, {"category": "Browser", "description": "Browse phosphorylation sites by species across 223 eukaryotes including 95 animals, 20 protists, 61 plants, and 48 fungi", "format": "http", "id": "epsd.browse", "name": "EPSD Browse by Species", "original_source": ["epsd"], "product_url": "https://epsd.biocuckoo.cn", "warnings": ["File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by SSLError(SSLCertVerificationError(1, '[SSL_ CERTIFICATE_VERIFY_FAILED] certificate verify failed_ certificate has expired (_ssl.c_1017)')))", "File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by NewConnectionError('<urllib3.connection.HTTPSConnection object at 0x7fd6e7ba1ee0>_ Failed to establish a new connection_ [Errno 111] Connection refused'))", "File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by NewConnectionError('<urllib3.connection.HTTPSConnection object at 0x7fe0f79e7820>_ Failed to establish a new connection_ [Errno 111] Connection refused'))", "File was not able to be retrieved when checked on 2025-11-25_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by SSLError(SSLCertVerificationError(1, '[SSL_ CERTIFICATE_VERIFY_FAILED] certificate verify failed_ certificate has expired (_ssl.c_1000)')))"]}, {"category": "Search", "description": "Advanced search interface supporting substrate search, peptide search, batch search, BLAST search, and multi-condition queries", "format": "http", "id": "epsd.search", "name": "EPSD Advanced Search", "original_source": ["epsd"], "product_url": "https://epsd.biocuckoo.cn", "warnings": ["File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by SSLError(SSLCertVerificationError(1, '[SSL_ CERTIFICATE_VERIFY_FAILED] certificate verify failed_ certificate has expired (_ssl.c_1017)')))", "File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by NewConnectionError('<urllib3.connection.HTTPSConnection object at 0x7fd6e7ba1a90>_ Failed to establish a new connection_ [Errno 111] Connection refused'))", "File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by NewConnectionError('<urllib3.connection.HTTPSConnection object at 0x7fe0f1bcc430>_ Failed to establish a new connection_ [Errno 111] Connection refused'))", "File was not able to be retrieved when checked on 2025-11-25_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by SSLError(SSLCertVerificationError(1, '[SSL_ CERTIFICATE_VERIFY_FAILED] certificate verify failed_ certificate has expired (_ssl.c_1000)')))"]}, {"category": "Download", "description": "Download page for complete phosphorylation site datasets and annotation data from EPSD 2.0", "format": "http", "id": "epsd.download", "name": "EPSD Data Download", "original_source": ["epsd"], "product_url": "https://epsd.biocuckoo.cn/Download.php", "secondary_source": [], "warnings": ["File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ /Download.php (Caused by SSLError(SSLCertVerificationError(1, '[SSL_ CERTIFICATE_VERIFY_FAILED] certificate verify failed_ certificate has expired (_ssl.c_1017)')))", "File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ /Download.php (Caused by NewConnectionError('<urllib3.connection.HTTPSConnection object at 0x7fd6e7e34050>_ Failed to establish a new connection_ [Errno 111] Connection refused'))", "File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ /Download.php (Caused by NewConnectionError('<urllib3.connection.HTTPSConnection object at 0x7fe0f79e4df0>_ Failed to establish a new connection_ [Errno 111] Connection refused'))", "File was not able to be retrieved when checked on 2025-11-25_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ /Download.php (Caused by SSLError(SSLCertVerificationError(1, '[SSL_ CERTIFICATE_VERIFY_FAILED] certificate verify failed_ certificate has expired (_ssl.c_1000)')))"]}, {"category": "GraphicalInterface", "description": "Interactive web interface for exploring and visualizing kinase-substrate interactions", "format": "http", "id": "kinace.portal", "name": "KinAce Web Portal", "original_source": ["phosphositeplus", "iptmnet", "uniprot", "epsd", "kinhub", "coralkinome", "darkkinasekb", "hgnc", "kegg", "interpro"], "product_url": "https://kinace.kinametrix.com/", "secondary_source": ["kinace"]}]
{"activity_status": "active", "category": "DataSource", "creation_date": "2025-11-13T00:00:00Z", "description": "EPSD (Eukaryotic Phosphorylation Site Database) is a comprehensive database that curates and annotates experimentally identified protein phosphorylation sites across eukaryotic species. Version 2.0 contains 2,769,163 phosphorylation sites in 362,707 phosphoproteins from 223 eukaryotic species (95 animals, 20 protists, 61 plants, 48 fungi). The database integrates data from high-throughput phosphoproteomic studies and 10 public databases, providing detailed annotations including phosphopeptide sequences, localization probability scores, cell/tissue sources, and primary references. EPSD 2.0 features 88,074 functional annotations for 32,762 phosphorylation sites, covering 58 types of downstream effects on phosphoproteins and 107 regulatory impacts on biological processes. For eight model organisms (human, mouse, rat, Drosophila, C. elegans, Arabidopsis, S. pombe, S. cerevisiae), phosphoproteins are meticulously annotated with information from 100 external resources across 15 aspects including kinase/phosphatase regulators, 3D structures, physicochemical characteristics, genomic variations, functional descriptions, protein domains, molecular interactions, drug-target associations, disease data, orthologs, transcript expression, proteomics, subcellular localization, and regulatory pathways. EPSD 2.0 represents a 2.5-fold increase in data volume compared to version 1.0 and provides intrinsic disorder propensity and surface accessibility calculations for phosphorylation sites. The database supports advanced search options including substrate search, peptide search, batch search, BLAST search, and browse by species functionality.", "domains": ["genomics", "proteomics"], "homepage_url": "https://epsd.biocuckoo.cn", "id": "epsd", "infores_id": "epsd", "last_modified_date": "2025-11-13T00:00:00Z", "layout": "resource_detail", "name": "Eukaryotic Phosphorylation Site Database 2.0", "products": [{"category": "Portal", "description": "Main web portal for EPSD 2.0 providing search, browse, and visualization of protein phosphorylation sites across 223 eukaryotic species with functional annotations", "format": "http", "id": "epsd.portal", "name": "EPSD 2.0 Portal", "original_source": ["epsd"], "product_url": "https://epsd.biocuckoo.cn", "warnings": ["File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by SSLError(SSLCertVerificationError(1, '[SSL_ CERTIFICATE_VERIFY_FAILED] certificate verify failed_ certificate has expired (_ssl.c_1017)')))", "File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by NewConnectionError('<urllib3.connection.HTTPSConnection object at 0x7fd6e7ba18b0>_ Failed to establish a new connection_ [Errno 111] Connection refused'))", "File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by NewConnectionError('<urllib3.connection.HTTPSConnection object at 0x7fe0f52798d0>_ Failed to establish a new connection_ [Errno 111] Connection refused'))", "File was not able to be retrieved when checked on 2025-11-25_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by SSLError(SSLCertVerificationError(1, '[SSL_ CERTIFICATE_VERIFY_FAILED] certificate verify failed_ certificate has expired (_ssl.c_1000)')))"]}, {"category": "Browser", "description": "Browse phosphorylation sites by species across 223 eukaryotes including 95 animals, 20 protists, 61 plants, and 48 fungi", "format": "http", "id": "epsd.browse", "name": "EPSD Browse by Species", "original_source": ["epsd"], "product_url": "https://epsd.biocuckoo.cn", "warnings": ["File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by SSLError(SSLCertVerificationError(1, '[SSL_ CERTIFICATE_VERIFY_FAILED] certificate verify failed_ certificate has expired (_ssl.c_1017)')))", "File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by NewConnectionError('<urllib3.connection.HTTPSConnection object at 0x7fd6e7ba1ee0>_ Failed to establish a new connection_ [Errno 111] Connection refused'))", "File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by NewConnectionError('<urllib3.connection.HTTPSConnection object at 0x7fe0f79e7820>_ Failed to establish a new connection_ [Errno 111] Connection refused'))", "File was not able to be retrieved when checked on 2025-11-25_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by SSLError(SSLCertVerificationError(1, '[SSL_ CERTIFICATE_VERIFY_FAILED] certificate verify failed_ certificate has expired (_ssl.c_1000)')))"]}, {"category": "Search", "description": "Advanced search interface supporting substrate search, peptide search, batch search, BLAST search, and multi-condition queries", "format": "http", "id": "epsd.search", "name": "EPSD Advanced Search", "original_source": ["epsd"], "product_url": "https://epsd.biocuckoo.cn", "warnings": ["File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by SSLError(SSLCertVerificationError(1, '[SSL_ CERTIFICATE_VERIFY_FAILED] certificate verify failed_ certificate has expired (_ssl.c_1017)')))", "File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by NewConnectionError('<urllib3.connection.HTTPSConnection object at 0x7fd6e7ba1a90>_ Failed to establish a new connection_ [Errno 111] Connection refused'))", "File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by NewConnectionError('<urllib3.connection.HTTPSConnection object at 0x7fe0f1bcc430>_ Failed to establish a new connection_ [Errno 111] Connection refused'))", "File was not able to be retrieved when checked on 2025-11-25_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ / (Caused by SSLError(SSLCertVerificationError(1, '[SSL_ CERTIFICATE_VERIFY_FAILED] certificate verify failed_ certificate has expired (_ssl.c_1000)')))"]}, {"category": "Download", "description": "Download page for complete phosphorylation site datasets and annotation data from EPSD 2.0", "format": "http", "id": "epsd.download", "name": "EPSD Data Download", "original_source": ["epsd"], "product_url": "https://epsd.biocuckoo.cn/Download.php", "secondary_source": [], "warnings": ["File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ /Download.php (Caused by SSLError(SSLCertVerificationError(1, '[SSL_ CERTIFICATE_VERIFY_FAILED] certificate verify failed_ certificate has expired (_ssl.c_1017)')))", "File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ /Download.php (Caused by NewConnectionError('<urllib3.connection.HTTPSConnection object at 0x7fd6e7e34050>_ Failed to establish a new connection_ [Errno 111] Connection refused'))", "File was not able to be retrieved when checked on 2025-12-04_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ /Download.php (Caused by NewConnectionError('<urllib3.connection.HTTPSConnection object at 0x7fe0f79e4df0>_ Failed to establish a new connection_ [Errno 111] Connection refused'))", "File was not able to be retrieved when checked on 2025-11-25_ Error connecting to URL_ HTTPSConnectionPool(host='epsd.biocuckoo.cn', port=443)_ Max retries exceeded with url_ /Download.php (Caused by SSLError(SSLCertVerificationError(1, '[SSL_ CERTIFICATE_VERIFY_FAILED] certificate verify failed_ certificate has expired (_ssl.c_1000)')))"]}, {"category": "GraphicalInterface", "description": "Interactive web interface for exploring and visualizing kinase-substrate interactions", "format": "http", "id": "kinace.portal", "name": "KinAce Web Portal", "original_source": ["phosphositeplus", "iptmnet", "uniprot", "epsd", "kinhub", "coralkinome", "darkkinasekb", "hgnc", "kegg", "interpro"], "product_url": "https://kinace.kinametrix.com/", "secondary_source": ["kinace"]}], "publications": [{"id": "PMID:40581078"}, {"id": "PMID:32008039"}], "synonyms": ["EPSD", "Eukaryotic phosphorylation site database", "EPSD 2.0"], "taxon": ["NCBITaxon:3702", "NCBITaxon:10090", "NCBITaxon:10116", "NCBITaxon:7227", "NCBITaxon:6239", "NCBITaxon:4932", "NCBITaxon:2759", "NCBITaxon:9606"]}