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DoRothEA is a curated resource of transcription factor (TF) – target gene (regulon) interactions with confidence scoring, enabling inference of TF activity from gene expression data. It integrates literature-curated interactions and various experimental evidence (e.g. ChIP-seq, TF binding motifs, perturbation data) to build context-agnostic and confidence-stratified regulons for multiple species. Frequently used with PROGENy to estimate pathway and TF activities.
No external IDs available
| ID | Name | URL | Category | Format | Description |
|---|---|---|---|---|---|
| dorothea.graph | DoRothEA Regulon Graph | v1.0.0 | Graph | r | Core TF–target regulon knowledge grap... |
| dorothea.r-package | DoRothEA R Package | dorothea.html | Process | http | Bioconductor R data package containin... |
| dorothea.docs | DoRothEA Documentation | dorothea.html | Documentation | http | Project documentation, usage examples... |
| ID | Name | URL | Category | Format | Relation | Description |
|---|---|---|---|---|---|---|
| bioteque.embeddings | Bioteque Embeddings | embeddings | Product | mixed | had primary source | Network embeddings of the Bioteque gr... |
| tflink.downloads | TFLink Downloads | download | Product | http | had primary source | Download page listing interaction tab... |
| tflink.interactions.human | TFLink Human Interactions | TFLink_Homo_sapiens_interactions_All_simpleFormat_v1.0.tsv.gz (295.1 MB) | Graph | tsv | had primary source | All TF-target gene interactions for H... |
| tflink.interactions.mouse | TFLink Mouse Interactions | TFLink_Mus_musculus_interactions_All_simpleFormat_v1.0.tsv.gz (166.9 MB) | Graph | tsv | had primary source | All TF-target gene interactions for M... |
| tflink.interactions.rat | TFLink Rat Interactions | TFLink_Rattus_norvegicus_interactions_All_simpleFormat_v1.0.tsv (12.4 MB) | Graph | tsv | had primary source | All TF-target gene interactions for R... |
| tflink.interactions.zebrafish | TFLink Zebrafish Interactions | TFLink_Danio_rerio_interactions_All_simpleFormat_v1.0.tsv (3.3 MB) | Graph | tsv | had primary source | All TF-target gene interactions for D... |
| tflink.interactions.fly | TFLink Fruit Fly Interactions | TFLink_Drosophila_melanogaster_interactions_All_simpleFormat_v1.0.tsv (53.9 MB) | Graph | tsv | had primary source | All TF-target gene interactions for D... |
| tflink.interactions.worm | TFLink Nematode Interactions | TFLink_Caenorhabditis_elegans_interactions_All_simpleFormat_v1.0.tsv (46.2 MB) | Graph | tsv | had primary source | All TF-target gene interactions for C... |
| tflink.interactions.yeast | TFLink Yeast Interactions | TFLink_Saccharomyces_cerevisiae_interactions_All_simpleFormat_v1.0.tsv (42.1 MB) | Graph | tsv | had primary source | All TF-target gene interactions for S... |
| tflink.interactions.human.mitab | TFLink Human Interactions (MITAB) | TFLink_Homo_sapiens_interactions_All_mitab_v1.0.tsv.gz (218.9 MB) | Graph | psi_mi_mitab | had primary source | All human TF-target gene interactions... |
| tflink.gmt.human | TFLink Human TF Target Gene Sets | TFLink_Homo_sapiens_interactions_All_GMT_ncbiGeneID_v1.0.gmt (36.1 MB) | Product | tsv | had primary source | Human gene sets, one per transcriptio... |
| tflink.binding-sites.human | TFLink Human Binding Sites | TFLink_Homo_sapiens_bindingSites_All_annotation_v1.0.tsv.gz (153.1 MB) | Product | tsv | had primary source | Genomic locations of human TF binding... |
| tflink.binding-sites.human.gff3 | TFLink Human Binding Sites (GFF3) | TFLink_Homo_sapiens_bindingSites_All_annotation_v1.0.gff3 (913.7 MB) | Product | gff | had primary source | Human TF binding site locations as a ... |
| tflink.binding-sites.human.fasta | TFLink Human Binding Site Sequences | TFLink_Homo_sapiens_bindingSites_All_v1.0.fasta (1.1 GB) | Product | fasta | had primary source | Nucleotide sequences of human TF bind... |
DoRothEA provides a curated, confidence-stratified collection of transcription factor (TF) to target gene interactions (regulons) across species. It aggregates multiple evidence sources (literature curation, ChIP-seq, in silico TF binding predictions, expression perturbation signatures) to support robust TF activity inference from transcriptomic data.
Often paired with PROGENy for complementary pathway and TF activity inference. Users subset regulons by confidence level (e.g., A–C) to balance coverage and specificity.
Please cite the 2019 Nature Communications paper and, where appropriate, the latest preprint/update.
Created: September 11, 2025 | Last modified: September 23, 2026