hrpimp

is a Data Source.

The Human Reference Protein Interactome Mapping Project (HRPIMP) is a systematic effort to map binary protein-protein interactions in human cells. The project produced the Human Reference Interactome (HuRI) database containing high-quality experimentally validated protein-protein interactions. Using systematic yeast two-hybrid (Y2H) screening approaches, HRPIMP produced proteome-scale human PPI datasets including HuRI, HI-union, and earlier CCSB interactome screens. The current HuRI portal provides the HuRI dataset with 52,569 interactions in TSV and PSI-MI formats, mapped to Ensembl identifiers for GENCODE protein-coding genes.

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Domains

proteomics, systems biology, biomedical, biological systems

License

CC BY 4.0

Homepage

hrpimp

Repository

GitHub

Infores ID

Unknown

FAIRsharing ID

Unknown

Product Summary

Products

From this Resource
ID Name URL Category Format Description
hrpimp.data HuRI Protein-Protein Interaction Data HuRI.tsv (1.6 MB) Product tsv Current HuRI TSV file containing 52,5...
hrpimp.huri.psi HuRI PSI-MI Interaction Data HuRI.psi (162.0 MB) Product psi_mi_mitab Current HuRI PSI-MI formatted interac...
hrpimp.web Interactome Atlas Web Portal www.interactome-atlas.org GraphicalInterface http Web interface for browsing and explor...
From other Resources
ID Name URL Category Format Relation Description
alzkb.browser AlzKB Graph Database Browser login GraphicalInterface http had primary source A browser interface for a knowledge g...
alzkb.data AlzKB Data Release (Version 2.0.0) v2.0.0 GraphProduct ❔ had primary source Memgraph data release for AlzKB.

Details

Human Reference Protein Interactome Mapping Project

The Human Reference Protein Interactome Mapping Project (HRPIMP) is a landmark systematic effort to comprehensively map binary protein-protein interactions (PPIs) in human cells. Led by the Center for Cancer Systems Biology at Dana-Farber Cancer Institute under principal investigators Marc Vidal and Frederick P. Roth, this project has produced the Human Reference Interactome (HuRI), one of the most comprehensive and high-quality maps of human protein interactions available. The current portal provides HuRI and related CCSB interactome datasets as simple TSV files and PSI-MI files.

Key Features

  • Systematic Y2H Screening: Uses yeast two-hybrid technology for high-throughput detection of binary protein-protein interactions
  • Comprehensive Coverage: Maps interactions for 9,094 human proteins
  • High-Quality Data: 64,006 experimentally validated binary protein-protein interactions
  • Open Access: Data freely available under Creative Commons BY 4.0 license
  • Reference Standard: Widely used reference resource for systems biology and disease research

Applications

The HuRI database and HRPIMP data have been integrated into numerous research projects and knowledge bases, including:

  • Alzheimer’s Disease research (AlzKB)
  • Cancer systems biology
  • Drug target identification
  • Disease mechanism studies
  • Pathway analysis
  • Network medicine

Data Products

The project provides downloadable TSV files containing protein-protein interaction data that can be integrated into research pipelines, knowledge graphs, and systems biology analyses.

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Created: November 19, 2025 | Last modified: June 02, 2026