is a Data Source.
L1000FWD (L1000 FireWorks Display) is a web application from the Ma'ayan Laboratory that visualizes over 16,000 drug and small-molecule induced gene expression signatures from the LINCS L1000 project as interactive two- and three-dimensional maps. Signatures can be colored by cell type, time point, concentration, mechanism of action, and clinical phase, and searched for mimicking or opposing signatures with up and down gene sets. Characteristic direction signatures, signature similarity matrices, gene sets, and metadata are available for download.
No external IDs available
| ID | Name | URL | Category | Format | Description |
|---|---|---|---|---|---|
| l1000fwd.portal ⚠ | L1000FWD Web Application | l1000fwd | Graphical Interface | http | Interactive fireworks and t-SNE maps ... |
| l1000fwd.api ⚠ | L1000FWD API | api_page | Programming Interface | json | REST API for searching drugs by name ... |
| l1000fwd.cd-signatures-landmark | L1000FWD CD Signatures (Landmark Genes) | CD_signatures_LM_42809x978.gctx (330.6 MB) | Product | hdf5 | Characteristic direction (CD) signatu... |
| l1000fwd.cd-signatures-full | L1000FWD CD Signatures (Full Space) | CD_signatures_full_42809x22268.gctx (7.1 GB) | Product | hdf5 | Characteristic direction (CD) signatu... |
| l1000fwd.adjacency-matrix | L1000FWD Signature Adjacency Matrix | Adjacency_matrix_LM_space_42809x42809.gctx (13.7 GB) | Product | hdf5 | Cosine similarity adjacency matrix am... |
| l1000fwd.gene-sets-gmt | L1000FWD Signature Gene Sets (GMT) | CD_signatures_binary_42809.gmt (115.0 MB) | Product | tsv | Up and down gene sets for the 42,809 ... |
| l1000fwd.gene-sets-json | L1000FWD Signature Gene Sets (JSON) | CD_signatures_binary_42809.json (169.5 MB) | Product | json | Up and down gene sets for the 42,809 ... |
| l1000fwd.signature-metadata | L1000FWD Signature Metadata | CD_signature_metadata.csv (4.8 MB) | Product | csv | Metadata for the L1000FWD signatures ... |
| l1000fwd.drug-metadata | L1000FWD Drug Metadata | Drugs_metadata.csv (7.4 MB) | Product | csv | Metadata for the profiled drugs and s... |
| l1000fwd.probes-landmark-metadata | L1000FWD Landmark Probe Metadata | Probes_L1000_metadata.csv (16.3 KB) | Product | csv | Metadata for the L1000 probes of the ... |
| l1000fwd.probes-full-metadata | L1000FWD Full Probe Metadata | Probes_full_metadata.csv (364.2 KB) | Product | csv | Metadata for the 22,268 probes used i... |
| ID | Name | URL | Category | Format | Relation | Description |
|---|---|---|---|---|---|---|
| drugmonizome.libraries ⚠ | Drugmonizome Drug Set Libraries | libraries | Product | json | was derived from | The 35 Drugmonizome drug set librarie... |
L1000FWD (L1000 FireWorks Display) is a web application from the Ma’ayan Laboratory at the Icahn School of Medicine at Mount Sinai. It lays out more than 16,000 drug and small-molecule induced gene expression signatures from the LINCS L1000 project as an interactive map, so that compounds with similar transcriptomic effects cluster together. The tool is meant for exploring mechanisms of action and for suggesting functions for less studied small molecules.
Signatures were computed with the characteristic direction (CD) method from L1000 profiles of human cell lines treated with small molecules. The downloadable set covers 42,809 significant signatures in both the 978 landmark gene space and the full 22,268-probe space, a cosine-similarity adjacency matrix among them, up and down gene sets in GMT and JSON form, and metadata for signatures, drugs, and probes. Per-cell-line t-SNE layouts and kNN graphs are offered for about 20 cell lines. Signature landing pages also show drug attributes such as mechanism of action and clinical phase, and prescription statistics (frequent diagnoses, co-prescribed drugs, age distribution) drawn from Mount Sinai Health System electronic medical records.
The web application supports signature similarity search with up and down gene sets, returning mimicking and opposing signatures, and a REST API exposes drug search, signature retrieval, gene set queries, and top-50 results. Bulk files are served from the download page. On 2026-10-19 the web application and API pages returned HTTP 503 while the static download files were still served.
The GitHub repository is released under CC BY-NC-SA 4.0. The repository README states that L1000FWD’s web tools and services are free for academic, non-profit use and that commercial users should contact Mount Sinai Innovation Partners for a license. Users are asked to cite Wang et al. 2018 (Bioinformatics).
Created: October 19, 2026 | Last modified: October 19, 2026