prism

is a Data Source.

PRISM (Profiling Relative Inhibition Simultaneously in Mixtures) is a high-throughput drug screening technology developed by the Broad Institute that measures the sensitivity of cancer cell lines to therapeutic compounds. PRISM uses DNA barcoding to pool hundreds of cell lines together, enabling massively parallel drug screening at unprecedented scale. The platform is integrated into the Cancer Dependency Map (DepMap) project and provides comprehensive dose-response data for drug-cancer cell line interactions.

Domains

drug discovery, pharmacology, biomedical, precision medicine, health

License

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Homepage

prism

Repository

GitHub

Infores ID

infores:prism

FAIRsharing ID

Unknown

Product Summary

Publications

Products

From this Resource
ID Name URL Category Format Description
prism.portal PRISM Data Portal prism GraphicalInterface http Web interface for exploring PRISM dru...
prism.datasets PRISM Drug Sensitivity Datasets data_page Product csv Downloadable drug sensitivity screeni...
prism.api DepMap API (PRISM data) api ProgrammingInterface http API access to PRISM drug screening data
From other Resources
ID Name URL Category Format Description
bioteque.embeddings Bioteque Embeddings embeddings Product ❔ Network embeddings of the Bioteque gr...

Details

PRISM

Overview

PRISM (Profiling Relative Inhibition Simultaneously in Mixtures) is a revolutionary high-throughput drug screening technology developed at the Broad Institute that enables massively parallel testing of therapeutic compounds against hundreds of cancer cell lines simultaneously. By leveraging DNA barcoding technology, PRISM can screen compounds at a scale and cost efficiency previously impossible, generating comprehensive drug sensitivity profiles across diverse cancer types.

Technology Platform

DNA Barcoding Approach

  • Each cell line is labeled with a unique DNA barcode
  • Cell lines are pooled together in a single well
  • After drug treatment, cell viability is measured by quantifying barcode abundance
  • Enables testing of 100+ cell lines per compound simultaneously

Screening Scale

  • Cell Lines: 500+ cancer cell lines representing diverse lineages
  • Compounds: Thousands of compounds including FDA-approved drugs and experimental molecules
  • Dose-Response: 8-point dose-response curves for accurate IC50 determination
  • Throughput: 10-100x more efficient than traditional one-cell-line-per-well approaches

Data Content

Drug Sensitivity Profiles

  • Comprehensive dose-response measurements
  • IC50 and AUC (Area Under Curve) values
  • Viability measurements across concentration ranges
  • Quality control metrics for each experiment

Cell Line Characterization

  • Genomic profiles (mutations, copy number)
  • Transcriptomic data (gene expression)
  • Lineage and subtype annotations
  • Integration with DepMap omics data

Compound Information

  • Chemical structures and properties
  • MOA (Mechanism of Action) annotations
  • Target information
  • Clinical trial status

Integration with DepMap

PRISM data is fully integrated into the Cancer Dependency Map project, enabling:

  • Correlation with CRISPR dependency data
  • Multi-omic biomarker discovery
  • Prediction of drug response
  • Identification of combination therapy opportunities

Applications

  • Drug Repurposing: Identifying new indications for approved drugs
  • Precision Medicine: Matching patients to therapies based on molecular features
  • Target Validation: Linking genetic dependencies to drug sensitivity
  • Biomarker Discovery: Identifying predictive markers of drug response
  • Combination Strategies: Finding synergistic drug combinations

Data Access

All PRISM data is freely available through:

  • DepMap Data Portal for bulk downloads
  • Interactive web interface for exploration and visualization
  • API access for programmatic queries
  • Regular quarterly data releases

Information Resource ID

This resource has the Information Resource identifier: infores:prism

License and Reuse

PRISM data is made available by the Broad Institute for research use through the DepMap portal, supporting open science and drug discovery efforts worldwide.

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Created: November 05, 2025 | Last modified: November 05, 2025